Integrated metabolomic and proteomics profiling reveals the promotion of Lactobacillus reuteri LR1 on amino acid metabolism in the gut-liver axis of weaned pigs.
Identifieur interne : 000314 ( Main/Exploration ); précédent : 000313; suivant : 000315Integrated metabolomic and proteomics profiling reveals the promotion of Lactobacillus reuteri LR1 on amino acid metabolism in the gut-liver axis of weaned pigs.
Auteurs : Hongbo Yi [République populaire de Chine] ; Guangda Yang [République populaire de Chine] ; Yunxia Xiong [République populaire de Chine] ; Qiwen Wu [République populaire de Chine] ; Hao Xiao [République populaire de Chine] ; Xiaolu Wen [République populaire de Chine] ; Xuefen Yang [République populaire de Chine] ; Li Wang [République populaire de Chine] ; Zongyong Jiang [République populaire de Chine]Source :
- Food & function [ 2042-650X ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- microbiologie : Suidae.
- métabolisme : Acides aminés, Foie, Suidae, Tube digestif.
- physiologie : Lactobacillus reuteri.
- Animaux, Métabolomique, Transcriptome.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Amino Acids.
- metabolism : Gastrointestinal Tract, Liver, Swine.
- microbiology : Swine.
- physiology : Lactobacillus reuteri.
- Animals, Metabolomics, Transcriptome.
Abstract
Lactobacillus reuteri LR1 improved growth performance of weaned pigs in our previous study. The objective of this study was to reveal effects of L. reuteri LR1 on amino acid (AA) metabolism in weaned pigs and its underlying mechanism using metabolomic and proteomics methods. Weaned pigs were fed a basal diet (CON) or the basal diet supplemented with 5 × 1010 cfu kg-1L. reuteri LR1 (LR1) for a 14 d period. Untargeted metabolomic analysis of the liver showed that LR1 up-regulated 33 metabolites and most of them were related to AA metabolism. Quantitative proteomics found that differential proteins were mainly involved in a metabolic process in the ileal mucosa of LR1 vs. CON. Integrated metabolomic and proteomics analysis showed that the LR1's enhancement of AA metabolism in the gut-liver axis is mediated by the up-regulated intestinal AA transporters in the pathway of protein digestion and absorption. Moreover, qPCR results confirmed that LR1 increased (p < 0.05) mRNA abundances of AA transporters (PepT1, EAAT3, rBAT, B0AT1, and b0,+AT) in the ileal mucosa compared with CON. Furthermore, western blot analysis showed that LR1 activated the mammalian target of the rapamycin complex 1 (mTORC1) signaling pathway by increasing the phosphorylation of S6 and 70S6K1 in the gut-liver axis of weaned pigs. Together, these data indicated that dietary supplemented LR1 enhanced AA metabolism by up-regulating intestinal AA transporter expression and activating the mTORC1 signaling pathway in the gut-liver axis of weaned pigs.
DOI: 10.1039/c9fo01781j
PubMed: 31651917
Affiliations:
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Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Yang, Guangda" sort="Yang, Guangda" uniqKey="Yang G" first="Guangda" last="Yang">Guangda Yang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Xiong, Yunxia" sort="Xiong, Yunxia" uniqKey="Xiong Y" first="Yunxia" last="Xiong">Yunxia Xiong</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Wu, Qiwen" sort="Wu, Qiwen" uniqKey="Wu Q" first="Qiwen" last="Wu">Qiwen Wu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Xiao, Hao" sort="Xiao, Hao" uniqKey="Xiao H" first="Hao" last="Xiao">Hao Xiao</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Wen, Xiaolu" sort="Wen, Xiaolu" uniqKey="Wen X" first="Xiaolu" last="Wen">Xiaolu Wen</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Yang, Xuefen" sort="Yang, Xuefen" uniqKey="Yang X" first="Xuefen" last="Yang">Xuefen Yang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Wang, Li" sort="Wang, Li" uniqKey="Wang L" first="Li" last="Wang">Li Wang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Jiang, Zongyong" sort="Jiang, Zongyong" uniqKey="Jiang Z" first="Zongyong" last="Jiang">Zongyong Jiang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31651917</idno><idno type="pmid">31651917</idno><idno type="doi">10.1039/c9fo01781j</idno><idno type="wicri:Area/Main/Corpus">000167</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000167</idno><idno type="wicri:Area/Main/Curation">000167</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000167</idno><idno type="wicri:Area/Main/Exploration">000167</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Integrated metabolomic and proteomics profiling reveals the promotion of Lactobacillus reuteri LR1 on amino acid metabolism in the gut-liver axis of weaned pigs.</title><author><name sortKey="Yi, Hongbo" sort="Yi, Hongbo" uniqKey="Yi H" first="Hongbo" last="Yi">Hongbo Yi</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Yang, Guangda" sort="Yang, Guangda" uniqKey="Yang G" first="Guangda" last="Yang">Guangda Yang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Xiong, Yunxia" sort="Xiong, Yunxia" uniqKey="Xiong Y" first="Yunxia" last="Xiong">Yunxia Xiong</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Wu, Qiwen" sort="Wu, Qiwen" uniqKey="Wu Q" first="Qiwen" last="Wu">Qiwen Wu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Xiao, Hao" sort="Xiao, Hao" uniqKey="Xiao H" first="Hao" last="Xiao">Hao Xiao</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Wen, Xiaolu" sort="Wen, Xiaolu" uniqKey="Wen X" first="Xiaolu" last="Wen">Xiaolu Wen</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Yang, Xuefen" sort="Yang, Xuefen" uniqKey="Yang X" first="Xuefen" last="Yang">Xuefen Yang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Wang, Li" sort="Wang, Li" uniqKey="Wang L" first="Li" last="Wang">Li Wang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Jiang, Zongyong" sort="Jiang, Zongyong" uniqKey="Jiang Z" first="Zongyong" last="Jiang">Zongyong Jiang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author></analytic><series><title level="j">Food & function</title><idno type="eISSN">2042-650X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acids (metabolism)</term><term>Animals (MeSH)</term><term>Gastrointestinal Tract (metabolism)</term><term>Lactobacillus reuteri (physiology)</term><term>Liver (metabolism)</term><term>Metabolomics (MeSH)</term><term>Swine (metabolism)</term><term>Swine (microbiology)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acides aminés (métabolisme)</term><term>Animaux (MeSH)</term><term>Foie (métabolisme)</term><term>Lactobacillus reuteri (physiologie)</term><term>Métabolomique (MeSH)</term><term>Suidae (microbiologie)</term><term>Suidae (métabolisme)</term><term>Transcriptome (MeSH)</term><term>Tube digestif (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Amino Acids</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Gastrointestinal Tract</term><term>Liver</term><term>Swine</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Suidae</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Swine</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acides aminés</term><term>Foie</term><term>Suidae</term><term>Tube digestif</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Lactobacillus reuteri</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Lactobacillus reuteri</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Metabolomics</term><term>Transcriptome</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Métabolomique</term><term>Transcriptome</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lactobacillus reuteri LR1 improved growth performance of weaned pigs in our previous study. The objective of this study was to reveal effects of L. reuteri LR1 on amino acid (AA) metabolism in weaned pigs and its underlying mechanism using metabolomic and proteomics methods. Weaned pigs were fed a basal diet (CON) or the basal diet supplemented with 5 × 10<sup>10</sup> cfu kg<sup>-1</sup>L. reuteri LR1 (LR1) for a 14 d period. Untargeted metabolomic analysis of the liver showed that LR1 up-regulated 33 metabolites and most of them were related to AA metabolism. Quantitative proteomics found that differential proteins were mainly involved in a metabolic process in the ileal mucosa of LR1 vs. CON. Integrated metabolomic and proteomics analysis showed that the LR1's enhancement of AA metabolism in the gut-liver axis is mediated by the up-regulated intestinal AA transporters in the pathway of protein digestion and absorption. Moreover, qPCR results confirmed that LR1 increased (p < 0.05) mRNA abundances of AA transporters (PepT1, EAAT3, rBAT, B<sup>0</sup>AT1, and b<sup>0,+</sup>AT) in the ileal mucosa compared with CON. Furthermore, western blot analysis showed that LR1 activated the mammalian target of the rapamycin complex 1 (mTORC1) signaling pathway by increasing the phosphorylation of S6 and 70S6K1 in the gut-liver axis of weaned pigs. Together, these data indicated that dietary supplemented LR1 enhanced AA metabolism by up-regulating intestinal AA transporter expression and activating the mTORC1 signaling pathway in the gut-liver axis of weaned pigs.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31651917</PMID><DateCompleted><Year>2020</Year><Month>04</Month><Day>16</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2042-650X</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>11</Issue><PubDate><Year>2019</Year><Month>Nov</Month><Day>01</Day></PubDate></JournalIssue><Title>Food & function</Title><ISOAbbreviation>Food Funct</ISOAbbreviation></Journal><ArticleTitle>Integrated metabolomic and proteomics profiling reveals the promotion of Lactobacillus reuteri LR1 on amino acid metabolism in the gut-liver axis of weaned pigs.</ArticleTitle><Pagination><MedlinePgn>7387-7396</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1039/c9fo01781j</ELocationID><Abstract><AbstractText>Lactobacillus reuteri LR1 improved growth performance of weaned pigs in our previous study. The objective of this study was to reveal effects of L. reuteri LR1 on amino acid (AA) metabolism in weaned pigs and its underlying mechanism using metabolomic and proteomics methods. Weaned pigs were fed a basal diet (CON) or the basal diet supplemented with 5 × 10<sup>10</sup> cfu kg<sup>-1</sup>L. reuteri LR1 (LR1) for a 14 d period. Untargeted metabolomic analysis of the liver showed that LR1 up-regulated 33 metabolites and most of them were related to AA metabolism. Quantitative proteomics found that differential proteins were mainly involved in a metabolic process in the ileal mucosa of LR1 vs. CON. Integrated metabolomic and proteomics analysis showed that the LR1's enhancement of AA metabolism in the gut-liver axis is mediated by the up-regulated intestinal AA transporters in the pathway of protein digestion and absorption. Moreover, qPCR results confirmed that LR1 increased (p < 0.05) mRNA abundances of AA transporters (PepT1, EAAT3, rBAT, B<sup>0</sup>AT1, and b<sup>0,+</sup>AT) in the ileal mucosa compared with CON. Furthermore, western blot analysis showed that LR1 activated the mammalian target of the rapamycin complex 1 (mTORC1) signaling pathway by increasing the phosphorylation of S6 and 70S6K1 in the gut-liver axis of weaned pigs. Together, these data indicated that dietary supplemented LR1 enhanced AA metabolism by up-regulating intestinal AA transporter expression and activating the mTORC1 signaling pathway in the gut-liver axis of weaned pigs.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yi</LastName><ForeName>Hongbo</ForeName><Initials>H</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-6719-7894</Identifier><AffiliationInfo><Affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Guangda</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiong</LastName><ForeName>Yunxia</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Qiwen</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiao</LastName><ForeName>Hao</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wen</LastName><ForeName>Xiaolu</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Xuefen</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Li</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Zongyong</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st street, Guangzhou 510640, China. jiangz28@qq.com wangli1@gdaas.cn yihongbo@gdaas.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>10</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Food Funct</MedlineTA><NlmUniqueID>101549033</NlmUniqueID><ISSNLinking>2042-6496</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000596">Amino Acids</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000596" MajorTopicYN="N">Amino Acids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D041981" MajorTopicYN="N">Gastrointestinal Tract</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D052200" MajorTopicYN="N">Lactobacillus reuteri</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008099" MajorTopicYN="N">Liver</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055432" MajorTopicYN="N">Metabolomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013552" MajorTopicYN="N">Swine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="N">Transcriptome</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>10</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>10</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31651917</ArticleId><ArticleId IdType="doi">10.1039/c9fo01781j</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Yi, Hongbo" sort="Yi, Hongbo" uniqKey="Yi H" first="Hongbo" last="Yi">Hongbo Yi</name></region><name sortKey="Jiang, Zongyong" sort="Jiang, Zongyong" uniqKey="Jiang Z" first="Zongyong" last="Jiang">Zongyong Jiang</name><name sortKey="Wang, Li" sort="Wang, Li" uniqKey="Wang L" first="Li" last="Wang">Li Wang</name><name sortKey="Wen, Xiaolu" sort="Wen, Xiaolu" uniqKey="Wen X" first="Xiaolu" last="Wen">Xiaolu Wen</name><name sortKey="Wu, Qiwen" sort="Wu, Qiwen" uniqKey="Wu Q" first="Qiwen" last="Wu">Qiwen Wu</name><name sortKey="Xiao, Hao" sort="Xiao, Hao" uniqKey="Xiao H" first="Hao" last="Xiao">Hao Xiao</name><name sortKey="Xiong, Yunxia" sort="Xiong, Yunxia" uniqKey="Xiong Y" first="Yunxia" last="Xiong">Yunxia Xiong</name><name sortKey="Yang, Guangda" sort="Yang, Guangda" uniqKey="Yang G" first="Guangda" last="Yang">Guangda Yang</name><name sortKey="Yang, Xuefen" sort="Yang, Xuefen" uniqKey="Yang X" first="Xuefen" last="Yang">Xuefen Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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